Composite

Part:BBa_K101015:Design

Designed by: Yiannis Kaznessis   Group: iGEM08_Minnesota   (2008-10-20)

Standard Promoter with RBS, GFP and terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 706


Design Notes

J23101 was digested with EcoRI and SPEI, and E0240 was digested with xBAI and PSTI and ligated to EcoRI and PSTI digested PSB3k3


Source

BioBricks

References